RPGR gene therapy presents challenges in cloning the coding sequence

UHC Presents Universal Challenges

Cloning and Religious Challenges

Genetic Engineering has conquered different arenas and life's various forms and caused drastic changes in them. Today, with the help of biotechnology and genetic engineering, mankind has reached high summits. One such summit is the decoding of human constituting particle which is the source of physical, behavioral features as well as intellectual power. This is nothing but the gene. Today man h...

Cloning and Sequence Analysis of Gene Encoding OipA from Iranian Clinical Helicobacter pylori

Background: Outer inflammatory protein A (OipA) is one of the important adhesins of H. pylori and a valuable candidate for vaccine development. Its gene is under "on-off" switch status which correlates with OipA protein expression. Objectives: We aimed to obtain a recombinant OipA clone (with "on" status) from an Iranian clinical isolate. Materials and Methods: A clinical H. pylori-isolate demo...

A Novel Human Lipid Binding Protein Coding Gene: PERF15, Sequence and Cloning

INTRODUCTION PERF15 is a testicular germ-cell specific fatty-acid binding protein (FABP) isolated from mammals, originally from rats. It encodes one of the most abundant proteins of rat spermatozoa localized in the perinuclear theca. Northern blot analysis has demonstrated that rat PERF15 mRNA is exclusively transcribed during meiosis and post-meiosis. In this study, we cloned and sequenced hum...

Isolation, Cloning and Sequence Analysis of 1-Aminocyclopropane-1-Carboxylate Deaminase Gene from Native Sinorhizobium meliloti

Background: Many plant growth-promoting bacteria including Rhizobia contain the 1-aminocyclopropane-1-carboxylate (ACC) deaminase enzyme that can leave ACC, and thereby lower the level of ethylene in stressed plants. Drought and salinity are the most common environmental stress factors for plants in Iran. Objectives: The main aim of this research was development of bio-fertilizers containing A...